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Lookup NU author(s): Dr Laura YoungORCiD, Dr Miguel Jarrin
This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).
© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.Graded refractive index lenses are inherent to advanced visual systems in animals. By understanding their formation and local optical properties, significant potential for improved ocular healthcare may be realized. We report a novel technique measuring the developing optical power of the eye lens, in a living animal, by exploiting the orthogonal imaging modality of a selective plane illumination microscope (SPIM). We have quantified the maturation of the lenticular refractive index at three different visible wavelengths using a combined imaging and ray tracing approach. We demonstrate that the method can be used with transgenic and vital dye labeling as well as with both fixed and living animals. Using a key eye lens morphogen and its inhibitor, we have measured their effects both on lens size and on refractive index. Our technique provides insights into the mechanisms involved in the development of this natural graded index micro-lens and its associated optical properties.
Author(s): Young LK, Jarrin M, Saunter CD, Quinlan RA, Girkin JM
Publication type: Article
Publication status: Published
Journal: Biomedical Optics Express
Year: 2018
Volume: 9
Issue: 5
Pages: 2176-2188
Print publication date: 01/05/2018
Online publication date: 10/04/2018
Acceptance date: 23/03/2018
Date deposited: 28/05/2020
ISSN (electronic): 2156-7085
Publisher: Optical Society of America
URL: https://doi.org/10.1364/BOE.9.002176
DOI: 10.1364/BOE.9.002176
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