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Designer artificial membrane binding proteins to direct stem cells to the myocardium

Lookup NU author(s): Professor Paul RaceORCiD

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Abstract

© 2019 The Royal Society of Chemistry. We present a new cell membrane modification methodology where the inherent heart tissue homing properties of the infectious bacteria Streptococcus gordonii are transferred to human stem cells. This is achieved via the rational design of a chimeric protein-polymer surfactant cell membrane binding construct, comprising the cardiac fibronectin (Fn) binding domain of the bacterial adhesin protein CshA fused to a supercharged protein. Significantly, the protein-polymer surfactant hybrid spontaneously inserts into the plasma membrane of stem cells without cytotoxicity, instilling the cells with a high affinity for immobilized fibronectin. Moreover, we show that this cell membrane reengineering approach significantly improves retention and homing of stem cells delivered either intracardially or intravenously to the myocardium in a mouse model.

Publication metadata

Author(s): Xiao W, Green TIP, Liang X, Delint RC, Perry G, Roberts MS, Le Vay K, Back CR, Ascione R, Wang H, Race PR, Perriman AW

Publication type: Article

Publication status: Published

Journal: Chemical Science

Year: 2019

Volume: 10

Issue: 32

Pages: 7610-7618

Print publication date: 28/08/2019

Online publication date: 03/07/2019

Acceptance date: 07/06/2019

ISSN (print): 2041-6520

ISSN (electronic): 2041-6539

Publisher: Royal Society of Chemistry

URL: https://doi.org/10.1039/c9sc02650a

DOI: 10.1039/c9sc02650a

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