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© 2014. Sustained forward migration through a fibrillar extracellular matrix requires localization of protrusive signals. Contact with fibronectin at the tip of a cell protrusion activates Rac1, and for linear migration it is necessary to dampen Rac1 activity in off-axial positions and redistribute Rac1 from non-protrusive membrane to the leading edge. Here, we identify interactions between coronin-1C (Coro1C), RCC2 and Rac1 that focus active Rac1 to a single protrusion. Coro1C mediates release of inactive Rac1 from non-protrusive membrane and is necessary for Rac1 redistribution to a protrusive tip and fibronectin-dependent Rac1 activation. The second component, RCC2, attenuates Rac1 activation outside the protrusive tip by binding to the Rac1 switch regions and competitively inhibiting GEF action, thus preventing off-axial protrusion. Depletion of Coro1C or RCC2 by RNA interference causes loss of cell polarity that results in shunting migration in 1D or 3D culture systems. Furthermore, morpholinos against Coro1C or RCC2, or mutation of any of the binding sites in the Rac1-RCC2- Coro1C complex delays the arrival of neural crest derivatives at the correct location in developing zebrafish, demonstrating the crucial role in migration guidance in vivo.
Author(s): Williamson RC, Cowell CAM, Hammond CL, Bergen DJM, Roper JA, Feng Y, Rendall TCS, Race PR, Bass MD
Publication type: Article
Publication status: Published
Journal: Journal of Cell Science
Year: 2014
Volume: 127
Issue: 19
Pages: 4292-4307
Print publication date: 01/10/2014
Acceptance date: 10/07/2014
ISSN (print): 0021-9533
ISSN (electronic): 1477-9137
Publisher: Company of Biologists Ltd
URL: https://doi.org/10.1242/jcs.154864
DOI: 10.1242/jcs.154864
PubMed id: 25074804
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