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Improving the success rate of protein crystallization by random microseed matrix screening

Lookup NU author(s): Professor Paul RaceORCiD

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Abstract

Random microseed matrix screening (rMMS) is a protein crystallization technique in which seed crystals are added to random screens. By increasing the likelihood that crystals will grow in the metastable zone of a protein's phase diagram, extra crystallization leads are often obtained, the quality of crystals produced may be increased, and a good supply of crystals for data collection and soaking experiments is provided. Here we describe a general method for rMMS that may be applied to either sitting drop or hanging drop vapor diffusion experiments, established either by hand or using liquid handling robotics, in 96-well or 24-well tray format.

Publication metadata

Author(s): Till M, Robson A, Byrne MJ, Nair AV, Kolek SA, Shaw Stewart PD, Race PR

Publication type: Article

Publication status: Published

Journal: JoVE

Year: 2013

Issue: 78

Online publication date: 31/08/2013

ISSN (electronic): 1940-087X

Publisher: JoVE

URL: https://doi.org/10.3791/50548

DOI: 10.3791/50548

PubMed id: 24022545

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