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Lookup NU author(s): Dr Tryfonia Mainou-Fowler, Dr Penelope Taylor, Professor Anne Dickinson, Professor Stephen Proctor
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The intracellular profiles of T helper type 1 (Th1) and T helper type 2 (Th2) T-cell cytokines by peripheral blood (PB) CD3(+) T-cells in patients with classical Hodgkin lymphoma (HL) has not been investigated before. The present study examines the cytoplasmic production of interleukin (IL) 2, 4, 10, tumour necrosis factor alpha (TNFalpha), and interferon gamma (IFNgamma) by activated PB CD3(+) T-cells and compares them with the profiles observed with normal individuals. We report a significantly lower mean level of intracellular IL2, TNFalpha and IFNgamma at any time post-cell activation in cells isolated from patients with HL compared with the normal control group. In contrast, the mean level of cytoplasmic IL4 was significantly higher in the HL compared with the control group. No significant difference between the two groups was observed with IL10. In the HL patient group, there was a significantly higher percentage of CD3(+)CD8(+) T-cells that synthesised IL4 compared with the CD3(+)CD4(+) sub-population, no such difference was observed in normal controls. The intensity of IL4 (expressed as relative median fluorescence) was significantly higher in the CD3(+)CD8(+) cells of the patients with HL compared with the CD3(+)CD4(+) sub-population, or with normal CD3(+)CD8(+) cells. In conclusion, there is reduced intracellular IL2, TNFalpha and IIFNgamma and increased cytoplasmic IL4 production by activated PB T-cells in patients with HL. The CD3(+)CD8(+) sub-population is responsible for the increased levels of IL4.
Author(s): Mainou-Fowler T, Taylor PRA, Miller S, Dickinson AM, Proctor SJ
Publication type: Article
Publication status: Published
Journal: Leukemia and Lymphoma
Year: 2003
Volume: 44
Issue: 8
Pages: 1325-1331
ISSN (print): 1042-8194
ISSN (electronic): 1026-8022
Publisher: Informa Healthcare
