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Browsing publications by Dr David Bolam.

Newcastle AuthorsTitleYearFull text
Dr David Bolam
Diverse events have transferred genes for edible seaweed digestion from marine to human gut bacteria2022
Declan Gray
Amy Glenwright
Dr Arnaud Basle
Carl Morland
Dr David Bolam
et al.
Insights into SusCD-mediated glycan import by a prominent gut symbiont2021
Dr David Bolam
A novel glycosidase plate-based assay for the quantification of galactosylation and sialylation on human IgG2020
Dr Lucy Crouch
Dr Arnaud Basle
Dr Chris Lamb
Dr Christopher Stewart
Kate Cooke
et al.
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown2020
Justina Briliūtė
Dr Elisabeth Lowe
Dr David Bolam
Dr Lucy Crouch
Complex N-glycan breakdown by gut Bacteroides involves an extensive enzymatic apparatus encoded by multiple co-regulated genetic loci2019
Dr Lucy Crouch
Dr David Bolam
Cloning, purification and biochemical characterisation of a GH35 beta-1,3/beta-1,6-galactosidase from the mucin-degrading gut bacterium Akkermansia muciniphila2018
Carl Morland
Dr David Bolam
Genetic variation of the SusC/SusD homologs from a polysaccharide utilization locus underlies divergent fructan specificities and functional adaptation in Bacteroides thetaiotaomicron strains2018
Dr David Bolam
Professor Bert van den Berg
TonB-dependent transport by the gut microbiota: novel aspects of an old problem2018
Dr Alan Cartmell
Dr Elisabeth Lowe
Dr Arnaud Basle
Dr Susan Firbank
Dr Didier Ndeh
et al.
How members of the human gut microbiota overcome the sulfation problem posed by glycosaminoglycans2017
Amy Glenwright
Dr Arnaud Basle
Dr Susan Firbank
Dr Hong Zheng
Dr David Bolam
et al.
Structural basis for nutrient acquisition by dominant members of the human gut microbiota2017
Dr Lucy Crouch
Dr Fiona Cuskin
Justina Briliūtė
Dr Arnaud Basle
Dr David Bolam
et al.
Degradation of complex N-glycans by gut Bacteroides species2016
Justina Briliūtė
Dr Lucy Crouch
Dr David Bolam
Insight into N-glycan breakdown by the gut microbiota2016
Dr Fiona Cuskin
Dr Lucy Crouch
Dr Arnaud Basle
Dr David Bolam
Emeritus Professor Harry Gilbert
et al.
Pivotal alpha mannosidase generates specificity for N-glycans through requirement for GlcNac at+2 subsite.2016
Dr David Bolam
Amy Glenwright
Structural basis for glycan acquisition by dominant members of the human gut microbiota2016
Dr Artur Rogowski
Jon Briggs
Dr Elisabeth Lowe
Dr Arnaud Basle
Carl Morland
et al.
Glycan complexity dictates microbial resource allocation in the large intestine2015
Dr Artur Rogowski
Dr Arnaud Basle
Dr Alexandra Solovyova
Emeritus Professor Harry Gilbert
Dr David Bolam
et al.
Evidence That GH115 alpha-Glucuronidase Activity, Which Is Required to Degrade Plant Biomass, Is Dependent on Conformational Flexibility2014
Dr David Bolam
The pattern of xylan acetylation suggests xylan may interact with cellulose microfibrils as a twofold helical screw in the secondary plant cell wall of Arabidopsis thaliana2014
Dr Elisabeth Lowe
Dr David Bolam
Tuning transcription of nutrient utilization genes to catabolic rate promotes growth in a gut bacterium2014
Dr Katalin Kovacs
Dr Adam Jackson
Dr David Bolam
Secretion and assembly of functional mini-cellulosomes from synthetic chromosomal operons in Clostridium acetobutylicum ATCC 8242013
Dr Sirintra Nakjang
Dr Didier Ndeh
Professor Anil Wipat
Dr David Bolam
Professor Robert Hirt
et al.
A Novel Extracellular Metallopeptidase Domain Shared by Animal Host-Associated Mutualistic and Pathogenic Microbes2012
Dr Elisabeth Lowe
Dr Arnaud Basle
Dr Susan Firbank
Dr David Bolam
A scissor blade-like closing mechanism implicated in transmembrane signaling in a Bacteroides hybrid two-component system2012
Dr David Bolam
Glycan recognition by the Bacteroidetes Sus-like systems2012
Dr David Bolam
Emeritus Professor Harry Gilbert
Novel Clostridium thermocellum Type I Cohesin-Dockerin Complexes Reveal a Single Binding Mode2012
Dr David Bolam
Mechanistic insight into polysaccharide use within the intestinal microbiota2011
Dr Elisabeth Lowe
Emeritus Professor Harry Gilbert
Dr David Bolam
Recognition and degradation of plant cell wall polysaccharides by two human gut symbionts2011
Cedric Montanier
Dr James Flint
Dr David Bolam
He Fang Xie
Ziyuan Liu
et al.
Circular Permutation Provides an Evolutionary Link between Two Families of Calcium-dependent Carbohydrate Binding Modules2010
Dr Susan Firbank
Dr David Bolam
Specificity of Polysaccharide Use in Intestinal Bacteroides Species Determines Diet-Induced Microbiota Alterations2010
Emeritus Professor Harry Gilbert
Dr David Bolam
Family 6 carbohydrate-binding modules display multiple beta 1,3-linked glucan-specific binding interfaces2009
Benedita Pinheiro
Emeritus Professor Harry Gilbert
Dr David Bolam
Functional insights into the role of novel type I cohesin and dockerin domains from Clostridium thermocellum2009
Cedric Montanier
Dr James Flint
Benedita Pinheiro
Carl Morland
Alan Cartmell
et al.
The Active Site of a Carbohydrate Esterase Displays Divergent Catalytic and Noncatalytic Binding Functions2009
Dr David Bolam
Dr Mark Proctor
Emeritus Professor Harry Gilbert
The crystal structure of two macrolide glycosyltransferases provides a blueprint for host cell antibiotic immunity2007
Dr James Flint
Dr David Bolam
Emeritus Professor Harry Gilbert
Differential recognition of plant cell walls by microbial xylan-specific carbohydrate-binding modules2006
Joanna Henshaw
Dr David Bolam
Emeritus Professor Harry Gilbert
Family 6 carbohydrate binding modules in β-agarases display exquisite selectivity for the non-reducing termini of agarose chains2006
Dr Mark Proctor
Dr David Bolam
Emeritus Professor Harry Gilbert
Insights into the Synthesis of Lipopolysaccharide and Antibiotics through the Structures of Two Retaining Glycosyltransferases from Family GT42006
Dr James Flint
Dr David Bolam
Emeritus Professor Harry Gilbert
Understanding the biological rationale for the diversity of cellulose-directed carbohydrate-binding modules in prokaryotic enzymes2006
Emeritus Professor Harry Gilbert
Dr David Bolam
Xyloglucan is recognized by carbohydrate-binding modules that interact with β-glucan chains2006
Dr Mark Proctor
Dr David Bolam
Emeritus Professor Harry Gilbert
Probing the breadth of macrolide glycosyltransferases: In vitro remodeling of a polyketide antibiotic creates active bacterial uptake and enhances potency2005
Dr James Flint
Dr David Bolam
Dr Edward Taylor
Emeritus Professor Harry Gilbert
Probing the mechanism of ligand recognition in family 29 carbohydrate-binding modules2005
Dr James Flint
Dr David Bolam
Dr Louise Tailford
Emeritus Professor Harry Gilbert
Structural dissection and high-throughput screening of mannosylglycerate synthase2005
Dr David Bolam
Gavin Pell
Emeritus Professor Harry Gilbert
Structure of a mannan-specific family 35 carbohydrate-binding module: Evidence for significant conformational changes upon ligand binding2005
Dr David Bolam
Emeritus Professor Harry Gilbert
Carbohydrate-binding modules: Fine-tuning polysaccharide recognition2004
Emeritus Professor Harry Gilbert
Dr David Bolam
Glycoside hydrolase carbohydrate-binding modules as molecular probes for the analysis of plant cell wall polymers2004
Dr James Flint
Emeritus Professor Harry Gilbert
Dr David Bolam
Ligand-mediated dimerization of a carbohydrate-binding module reveals a novel mechanism for protein-carbohydrate recognition2004
Dr David Bolam
Emeritus Professor Harry Gilbert
Reduction of starch granule size by expression of an engineered tandem starch-binding domain in potato plants2004
Joanna Henshaw
Dr David Bolam
Emeritus Professor Harry Gilbert
The crystal structure of the family 6 carbohydrate binding module from Cellvibrio mixtus endoglucanase 5A in complex with oligosaccharides reveals two distinct binding sites with different ligand specificities2004
Dr David Bolam
Emeritus Professor Harry Gilbert
The family 11 carbohydrate-binding module of Clostridium thermocellum Lic26A-Cel5E accommodates β-1,4- and β-1,3-1,4-mixed linked glucans at a single binding site2004
Joanna Henshaw
Dr David Bolam
Emeritus Professor Harry Gilbert
The family 6 carbohydrate binding module CmCBM6-2 contains two ligand-binding sites with distinct specificities2004
Dr David Bolam
Gavin Pell
Deborah Hogg
Greta Galbraith
Emeritus Professor Harry Gilbert
et al.
X4 modules represent a new family of carbohydrate-binding modules that display novel properties2004
Gavin Pell
Huamao Du
Emeritus Professor Harry Gilbert
Dr David Bolam
Importance of hydrophobic and polar residues in ligand binding in the family 15 carbohydrate-binding module from Cellvibrio japonicus Xyn10C2003
Tibor Nagy
Professor Jeremy Lakey
Dr David Bolam
Emeritus Professor Harry Gilbert
The α-Glucuronidase, GlcA67A, of Cellvibrio japonicus utilizes the carboxylate and methyl groups of aldobiouronic acid as important substrate recognition determinants2003
Emeritus Professor Harry Gilbert
Dr David Bolam
Calcium binding and thermostability of carbohydrate binding module CBM4-2 of Xyn10A from Rhodothermus marinus2002
Dr David Bolam
Lorand Szabo
Dr Vincent McKie
Emeritus Professor Harry Gilbert
Promiscuity in ligand-binding: The three-dimensional structure of a Piromyces carbohydrate-binding module, CBM29-2, in complex with cello-and mannohexaose2002
Dr David Bolam
Emeritus Professor Harry Gilbert
A Novel Carbohydrate-binding Protein Is a Component of the Plant Cell Wall-degrading Complex of Piromyces equi2001
He Fang Xie
Emeritus Professor Harry Gilbert
Dr David Bolam
Clostridium thermocellum Xyn10B carbohydrate-binding module 22-2: The role of conserved amino acids in ligand binding2001
Deborah Hogg
Dr David Bolam
Dr Vincent McKie
Emeritus Professor Harry Gilbert
Crystal Structure of Mannanase 26A from Pseudomonas cellulosa and Analysis of Residues Involved in Substrate Binding2001
Dr David Bolam
Emeritus Professor Harry Gilbert
Evidence for synergy between family 2b carbohydrate binding modules in Cellulomonas fimi Xylanase 11A2001
Lorand Szabo
He Fang Xie
Dr David Bolam
Emeritus Professor Harry Gilbert
Structure of a Family 15 Carbohydrate-binding Module in Complex with Xylopentaose2001
Lorand Szabo
He Fang Xie
Dr David Bolam
Emeritus Professor Harry Gilbert
Structure of a family 15 carbohydrate-binding module in complex with xylopentaose: Evidence that xylan binds in an approximate 3-fold helical conformation2001
Dr David Bolam
Emeritus Professor Harry Gilbert
The Location of the Ligand-binding Site of Carbohydrate-binding Modules That Have Evolved from a Common Sequence Is Not Conserved2001
He Fang Xie
Dr David Bolam
Emeritus Professor Harry Gilbert
The structural basis for the ligand specificity of family 2 carbohydrate-binding modules2000
Dr David Bolam
Emeritus Professor Harry Gilbert
The X6 'thermostabilizing' domains of xylanases are carbohydrate-binding modules: Structure and biochemistry of the Clostridium thermocellum X6b domain2000
Dr David Bolam
Emeritus Professor Harry Gilbert
A family IIb xylan-binding domain has a similar secondary structure to a homologous family IIa cellulose-binding domain but different ligand specificity1999
Dr David Bolam
Emeritus Professor Harry Gilbert
The type II and X cellulose-binding domains of Pseudomonas xylanase A potentiate catalytic activity against complex substrates by a common mechanism1999
Dr David Bolam
Emeritus Professor Harry Gilbert
Pseudomonas cellulose-binding domains mediate their effects by increasing enzyme substrate proximity1998
Dr David Bolam
Emeritus Professor Harry Gilbert
Cellulose binding domains and linker sequences potentiate the activity of hemicellulases against complex substrates1997
Dr David Bolam
Dr Richard Virden
Professor Jeremy Lakey
Emeritus Professor Harry Gilbert
Mannanase A from Pseudomonas fluorescens ssp. cellulosa is a retaining glycosyl hydrolase in which E212 and E320 are the putative catalytic residues1996