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Lookup NU author(s): Dr Michael Laker, Professor Andrew Pearson
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Mannitol, 3-O-methylglucose and lactulose administered orally are used to investigate small intestinal absorption pathways and mucosal integrity. Current methods of analysis include thin-layer chromatography, gas chromatography (GC) and enzymatic analysis, which require separate estimation of mono- and disaccharides and for GC, prior derivatization. We describe a high-pressure anion-exchange chromatographic method coupled with pulsed electrochemical detection allowing simultaneous measurement of all three sugars and its clinical application in monitoring intestinal damage in human immunodeficiency virus (HIV) infection. Sample preparation is simple and fast. All sugars are resolved within 10 min. Mean recovery is 93.3% for all sugars and the overall relative standard deviation is 4.2%. Intestinal permeability (lactulose/mannitol ratio) rises with disease progression to AIDS, indicating mucosal damage. The greatest increase in permeability is associated with chronic diarrhoea. The method is an ideal non-invasive test to assess gut mucosal damage in HIV infection.
Author(s): Fleming SC, Kynaston JA, Laker MF, Pearson ADJ, Kapembwa MS, Griffin GE
Publication type: Conference Proceedings (inc. Abstract)
Publication status: Published
Conference Name: 1992 International ION Chromatography Symposium
Year of Conference: 1993
Pages: 293-297
Publisher: Journal of Chromatography A: Elsevier BV
URL: http://dx.doi.org/10.1016/0021-9673(93)80193-C
DOI: 10.1016/0021-9673(93)80193-C
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