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Application of a sensitive immunoassay to the study of DNA adducts formed in peripheral blood mononuclear cells of patients undergoing high-dose melphalan therapy

Lookup NU author(s): Dr Michael Tilby, Professor Herbie Newell


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The levels of DNA adducts formed in peripheral blood mononuclear cells of 13 patients undergoing high-dose melphalan therapy were determined 0-24 h after drug administration using a modification of a previously described immunoassay. This assay was validated for DNA extracted from drug-treated cells. Adduct levels in normal mononuclear blood cells 1 h after drug administration correlated well (r = 0.846) with drug dose (expressed as mg/m2) and with area under the curve for plasma levels of melphalan during the first h (r = 0.842). 1 patient sustained a high degree of toxic side-effects from the melphalan treatment and showed a high level of adducts. Plasma cell leukaemia tumour cells from another patient showed a level of adducts approximately six times higher than those in the normal blood cells of the other patients. The levels of DNA adducts in normal peripheral blood mononuclear cells did not change markedly between 1 and 24 h after drug administration.

Publication metadata

Author(s): Tilby, M.J., Newell, D.R., Viner, C., Selby, P.J., Dean, C.J.

Publication type: Article

Publication status: Unknown

Journal: European Journal of Cancer

Year: 1993

Volume: 29A

Issue: 5

Pages: 681-686

Print publication date: 01/01/1993

ISSN (print): 0959-8049

ISSN (electronic): 1879-0852


DOI: 10.1016/S0959-8049(05)80346-6


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