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OCT embedded sections of pathological specimens as a source of high quality RNA for reverse transcriptase/polymerase chain reaction

Lookup NU author(s): Professor Julie Irving, Gerard Cain, Dr Brian Angus

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Abstract

OCT embedded cryostat sections of stored pathological specimens of non-Hodgkin's lymphoma were used to provide RNA. After reverse transcription to produce cDNA, the polymerase chain reaction was performed with primers for standard and variant forms of the CD44 molecule. Using Southern transfer and hybridisation with a probe specific for exon 4 of the CD44 gene, both standard and variant forms were visualised by autoradiography. This method was shown to be applicable to other gene products by using primers specific for the abl and bcr genes. This technique permits retrospective analysis of RNA from small amounts of stored pathological samples.


Publication metadata

Author(s): Irving JAE, Cain G, Parr A, Howard M, Angus B, Cattan AR

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Pathology

Year: 1996

Volume: 49

Issue: 3

Pages: 258-259

Print publication date: 01/03/1996

ISSN (print): 0021-9746

ISSN (electronic): 1472-4146

URL: http://dx.doi.org/10.1136/jcp.49.3.258

DOI: 10.1136/jcp.49.3.258

PubMed id: 8675742


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