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Lookup NU author(s): Professor Julie Irving,
Dr Brian Angus
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OCT embedded cryostat sections of stored pathological specimens of non-Hodgkin's lymphoma were used to provide RNA. After reverse transcription to produce cDNA, the polymerase chain reaction was performed with primers for standard and variant forms of the CD44 molecule. Using Southern transfer and hybridisation with a probe specific for exon 4 of the CD44 gene, both standard and variant forms were visualised by autoradiography. This method was shown to be applicable to other gene products by using primers specific for the abl and bcr genes. This technique permits retrospective analysis of RNA from small amounts of stored pathological samples.
Author(s): Irving JAE, Cain G, Parr A, Howard M, Angus B, Cattan AR
Publication type: Article
Publication status: Published
Journal: Journal of Clinical Pathology
Print publication date: 01/03/1996
ISSN (print): 0021-9746
ISSN (electronic): 1472-4146
PubMed id: 8675742
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