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Phototoxicity of Rose Bengal in mycological media - implications for laboratory practice

Lookup NU author(s): Dr John Perry


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The effects of Rose Bengal (RB) on plate counts of the bacteria Escherichia coli and Pseudomonas aeruginosa, and the yeast Saccharomyces cerevisiae, were studied under natural sunlight and artificial fluorescent lighting. While RE was not inherently toxic in darkness at concentrations found in mycological media, the illumination of media containing RE caused a decrease in colony counts in all cases, and especially for surface spread plates. A negative synergy was observed between chloramphenicol, RE and illumination using a spring water sample containing substantial numbers of Gram-negative bacteria. Exposure of media containing RE to moderate amounts of light during standard laboratory procedures may inhibit microbial growth, with positive benefits in relation to the suppression of contaminant bacteria, or negative implications where fungi are inhibited.

Publication metadata

Author(s): Chilvers KF, Reed RH, Perry JD

Publication type: Article

Publication status: Published

Journal: Letters in Applied Microbiology

Year: 1999

Volume: 28

Issue: 2

Pages: 103-107

Print publication date: 01/02/1999

ISSN (print): 0266-8254

ISSN (electronic): 1472-765X

Publisher: Wiley-Blackwell Publishing Ltd.


DOI: 10.1046/j.1365-2672.1999.00492.x


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