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Lookup NU author(s): Dr John Perry, Professor Kate Gould
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Two rapid (1-h) assays for the detection of Staphylococcus aureus staphylocoagulase were developed by using the fluorogenic thrombin substrates N-t-boc-Val-Pro-Arg-7-amido-4-methylcoumarin (VPA) and N-t-boc-beta-benzyl-Asp-Pro-Arg-7-amido-4-methylocoumarin (BB), The assays were compared to the tube coagulase test and latex agglutination (LA) (Sanofi Diagnostics Pasteur, Guildford, Surrey, United Kingdom) by using 406 clinical isolates of staphylococci, and they produced positive and negative predictive values of 99.2 and 99.1% for LA, 98.9 and 92.7% for VPA, and 98.9 and 99.1% for BB. Fluorescent assays used colonies from solid media, thereby eliminating the need far broth cultures, and were performed in microtiter trays, thus making them suitable for large-scale screening.
Author(s): Holliday MG, Ford M, Perry JD, Gould FK
Publication type: Article
Publication status: Published
Journal: Journal of Clinical Microbiology
Year: 1999
Volume: 37
Issue: 4
Pages: 1190-1192
Print publication date: 01/04/1999
ISSN (print): 0095-1137
ISSN (electronic): 1070-633X
Publisher: American Society for Microbiology
