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Rapid identification of Staphylococcus aureus by using fluorescent staphylocoagulase assays

Lookup NU author(s): Dr John Perry, Professor Kate Gould


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Two rapid (1-h) assays for the detection of Staphylococcus aureus staphylocoagulase were developed by using the fluorogenic thrombin substrates N-t-boc-Val-Pro-Arg-7-amido-4-methylcoumarin (VPA) and N-t-boc-beta-benzyl-Asp-Pro-Arg-7-amido-4-methylocoumarin (BB), The assays were compared to the tube coagulase test and latex agglutination (LA) (Sanofi Diagnostics Pasteur, Guildford, Surrey, United Kingdom) by using 406 clinical isolates of staphylococci, and they produced positive and negative predictive values of 99.2 and 99.1% for LA, 98.9 and 92.7% for VPA, and 98.9 and 99.1% for BB. Fluorescent assays used colonies from solid media, thereby eliminating the need far broth cultures, and were performed in microtiter trays, thus making them suitable for large-scale screening.

Publication metadata

Author(s): Holliday MG, Ford M, Perry JD, Gould FK

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Microbiology

Year: 1999

Volume: 37

Issue: 4

Pages: 1190-1192

Print publication date: 01/04/1999

ISSN (print): 0095-1137

ISSN (electronic): 1070-633X

Publisher: American Society for Microbiology