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Lookup NU author(s): Emeritus Professor Calum McNeilORCiD, Dr Jeanette Pritchard, Professor Ronald Armstrong, Dr Dale Athey
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Enzyme-catalyzed polymer transformation with electrochemical ac impedance detection has been employed for the measurement of urea and creatinine in serum samples. A polymer, based on poly(methylvinyl ether)/maleic anhydride modified by esterification with n-octanol, which is stable at pH 7.4 and which is transformed rapidly in response to alkaline pH changes, was linked to enzymatic reactions between urease and urea or creatinine deiminase and creatinine to produce a disposable sensor system. The polymer was screen-printed onto interdigitated screen-printed carbon electrodes and the electrodes overlaid with absorbent pads containing the relevant enzyme. Application of serum samples, "spiked" with either urea or creatinine, resulted in rapid polymer transformation, and resultant changes in the capacitance of the polymer-coated electrodes were analyte-concentration dependent. Additional information on the mechanisms of polymer transformation was obtained from dynamic quartz crystal microbalance measurements.
Author(s): Ho WO, Krause S, McNeil CJ, Pritchard JA, Armstrong RD, Athey D, Rawson K
Publication type: Article
Publication status: Published
Journal: Analytical Chemistry
Year: 1999
Volume: 71
Issue: 10
Pages: 1940-1946
Print publication date: 17/04/1999
ISSN (print): 0003-2700
ISSN (electronic): 1520-6882
Publisher: American Chemical Society
URL: http://dx.doi.org/10.1021/ac981367d
DOI: 10.1021/ac981367d
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