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Electrochemical sensor for measurement of urea and creatinine in serum based on ac impedance measurement of enzyme-catalyzed polymer transformation

Lookup NU author(s): Emeritus Professor Calum McNeilORCiD, Dr Jeanette Pritchard, Professor Ronald Armstrong, Dr Dale Athey


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Enzyme-catalyzed polymer transformation with electrochemical ac impedance detection has been employed for the measurement of urea and creatinine in serum samples. A polymer, based on poly(methylvinyl ether)/maleic anhydride modified by esterification with n-octanol, which is stable at pH 7.4 and which is transformed rapidly in response to alkaline pH changes, was linked to enzymatic reactions between urease and urea or creatinine deiminase and creatinine to produce a disposable sensor system. The polymer was screen-printed onto interdigitated screen-printed carbon electrodes and the electrodes overlaid with absorbent pads containing the relevant enzyme. Application of serum samples, "spiked" with either urea or creatinine, resulted in rapid polymer transformation, and resultant changes in the capacitance of the polymer-coated electrodes were analyte-concentration dependent. Additional information on the mechanisms of polymer transformation was obtained from dynamic quartz crystal microbalance measurements.

Publication metadata

Author(s): Ho WO, Krause S, McNeil CJ, Pritchard JA, Armstrong RD, Athey D, Rawson K

Publication type: Article

Publication status: Published

Journal: Analytical Chemistry

Year: 1999

Volume: 71

Issue: 10

Pages: 1940-1946

Print publication date: 17/04/1999

ISSN (print): 0003-2700

ISSN (electronic): 1520-6882

Publisher: American Chemical Society


DOI: 10.1021/ac981367d


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