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Lookup NU author(s): Emeritus Professor Brian Diffey
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We have carried out ex vivo studies to examine the feasibility of using fluorescence spectroscopy as an in vivo quantitative technique to assess sunscreen substantivity in terms of skin surface thickness and/or photoprotection. We found that the majority of sunscreens produced insufficient natural fluorescence and so we have attempted to increase the fluorescent signal by adding various fluorescing agents to the sunscreens. However, none of these substances is ideal; either they do not bind sufficiently strongly to sunscreen products, or their fluorescence is quenched by the active ingredients contained within sunscreens. The feasibility of using fluorescence spectroscopy for in vivo quantitative assessments of sunscreen substantivity therefore remains unproved and is dependent on a suitable fluorescent agent being found. Such an agent would have to be non-toxic, mix readily with sunscreens and be excited by visible wavelengths. (C) 1999 Elsevier Science S.A. All rights reserved.
Author(s): Stokes RP, Diffey BL
Publication type: Article
Publication status: Published
Journal: Journal of Photochemistry and Photobiology, B: Biology
Print publication date: 01/11/1999
ISSN (print): 1011-1344
ISSN (electronic): 1873-2682
Publisher: Elsevier SA
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