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To accurately characterize the carbohydrate moieties of oligosaccharide chains in glycosylated proteins, it is necessary to distinguish exactly which types of oligosaccharides are present at which site. We describe lectin overlay assays, which take advantage of the ability of lectins to distinguish between different types of glycoproteins via recognition of terminal sugars, thus allowing the chain type and peripheral antigenic components to be determined. Three microassays involving lectins are reported in this paper: non-protease treated intact glycoproteins; glycopeptides released by prior digestion of the glycoprotein and then separated by HPLC; and release of sugars from glycoproteins by hydrazinolysis and then coupling them to a multivalent support.
Author(s): Wong NKC, Kanu N, Thandrayen N, Rademaker GJ, Baldwin CI, Renouf DV, Hounsell EF
Publication type: Article
Publication status: Published
Journal: Molecular Biotechnology
Year: 2000
Volume: 14
Issue: 2
Pages: 147-155
ISSN (print): 1073-6085
ISSN (electronic): 1559-0305
Publisher: Humana Press
URL: http://dx.doi.org/10.1385/MB:14:2:147
DOI: 10.1385/MB:14:2:147
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