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Alafosfalin as a selective agent for isolation of Salmonella from clinical samples

Lookup NU author(s): Dr John Perry, Professor Kate Gould


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The selectivity of a range of culture media for the detection of Salmonella was assessed using 435 strains of gram-negative bacteria. These media showed limited ability to inhibit non-Salmonella strains found in stool samples. We report the evaluation of alafosfalin as a selective agent for isolation of Salmonella from stool samples. Susceptibility studies with this agent showed that non-typhi Salmonella strains were relatively resistant (mean MIC, 10.2 mg/liter) compared to many coliforms including Escherichia coli (mean MIC, 0.7 mg/liter). A chromogenic medium, ABC medium, was modified to incorporate alafosfalin and was compared,with standard ABC medium and Hektoen enteric agar for the isolation of Salmonella from 1,000 stool samples. On direct culture, modified ABC medium showed higher recovery of Salmonella (53.6%) compared with either ABC medium (35.7%) or Hektoen enteric agar (48.2%). We conclude that alafosfalin is a useful selective agent for the isolation of Salmonella from stool samples.

Publication metadata

Author(s): Perry JD, Riley G, Gould FK, Perez JM, Boissier E, Ouedraogo RT, Freydiere AM

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Microbiology

Year: 2002

Volume: 40

Issue: 11

Pages: 3913-3916

ISSN (print): 0095-1137

ISSN (electronic): 1098-660X

Publisher: American Society for Microbiology


DOI: 10.1128/JCM.40.11.3913-3916.2002


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