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Lookup NU author(s): Professor Volker Straub
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Increased sarcolemmal permeability has been implicated as a major pathological event in muscular dystrophies. In our study. we evaluated whether damaged muscle fibres can be specifically targeted using albumin as a carrier. We tagged human serum albumin (HSA) with Gadolinium (Gd) and systemically applied this compound (Gd-DTPA-HSA) to wildtype and dystrophin-deficient mdx mice. We performed magnetic resonance imaging before and after intravenous administration of Gd-DTPA-HSA and found localised signal enhancement only in mdx skeletal muscle. We also examined skeletal muscle after contrast enhanced magnetic resonance imaging using anti-human albumin antibodies and demonstrated intracellular accumulation of Gd-DTPA-HSA in clusters of damaged mdx muscle fibres. Comparison of magnetic resonance imaging and histological data emphasised the value of contrast agent enhanced magnetic resonance imaging for the in vivo assessment of fibre damage in muscular dystrophies. Furthermore. our data provide evidence that albumin can be used as a carrier to target covalently bound molecules to degenerating muscle fibres. (C) 2004 Elsevier B.V. All rights reserved.
Author(s): Amthor H, Egelhof T, McKinnell I, Ladd ME, Janssen I, Weber J, Sinn H, Schrenk HH, Forsting M, Voit T, Straub V
Publication type: Article
Publication status: Published
Journal: Neuromuscular Disorders
ISSN (print): 0960-8966
ISSN (electronic): 1873-2364
Publisher: Elsevier B.V.
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