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Improved method for immunoperoxidase detection of membrane antigens in frozen sections

Lookup NU author(s): Professor Archibald Malcolm, Professor Andrew Pearson

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Abstract

The visualisation of membrane antigens in frozen sections using metal-enhanced 3,3'diaminobenzidine (DAB) was investigated. Particular attention was paid to the degree of reaction enhancement, the disruptive effect on morphological detail, and the ease of the techniques. The best result was obtained using nickel-modified DAB at pH 6.0 to develop the peroxidase enzyme, with further enhancement in cobalt chloride at neutral pH. Silver methenamine enhancement is also possible but can give rise to non-specific or background staining.

Publication metadata

Author(s): Green MA, Sviland L, Malcolm AJ, Pearson ADJ

Publication type: Article

Publication status: Published

Journal: Journal of Clinical Pathology

Year: 1989

Volume: 42

Issue: 8

Pages: 875-880

Print publication date: 01/08/1989

ISSN (print): 0021-9746

ISSN (electronic): 1472-4146

URL: http://dx.doi.org/10.1136/jcp.42.8.875

DOI: 10.1136/jcp.42.8.875

PubMed id: 2671055

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