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CD59a deficient mice display reduced B cell activity and antibody production in response to T-dependent antigens

Lookup NU author(s): Dr Rachel Davies, Professor Kevin Marchbank

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Abstract

CD59a is the primary regulator of membrane attack complex in mice. Recently, we have shown that CD59a-deficient (Cd59a-/-) mice exhibit enhanced CD4+ T cell responses. Here, we explored the effects of CD59a on B cell function and antibody production. Contrary to our expectations, Cd59a-/- mice showed a decreased humoral immune response to a T cell dependent antigen, sheep red blood cells. We found that the decreased humoral immune response was associated with a reduction in plasma cell number in vivo and reduced ability to respond to stimuli during in vitro culture experiments. Using MLR studies in which purified wild type or Cd59a-/- CD4+ T cells were mixed with purified B cells from each source, we found that the reduced B cell activation was largely due to the absence of CD59a on CD4+ T cells. Furthermore, a CD59a fusion protein bound specifically to mouse B cells, and enhanced B cell proliferation in a MLR, demonstrating that B cells express an as yet unidentified ligand for CD59a that aids in B cell activation.


Publication metadata

Author(s): Sivasankar B, Donev RM, Longhi MP, Hughes TR, Davies R, Cole DS, Morgan BP, Marchbank KJ

Publication type: Article

Publication status: Published

Journal: Molecular Immunology

Year: 2007

Volume: 44

Issue: 11

Pages: 2978-2987

ISSN (print): 0161-5890

ISSN (electronic): 1872-9142

Publisher: Pergamon

URL: http://dx.doi.org/10.1016/j.molimm.2006.12.025

DOI: 10.1016/j.molimm.2006.12.025

Notes: 0161-5890 (Print) Journal Article Research Support, Non-U.S. Gov't


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