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Lookup NU author(s): Dr Glyn NelsonORCiD
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Functional activity of N- and C-terminal fluorescent fusion proteins between STAT6 and EGFP was demonstrated through IL-4-dependent transcriptional activation and nuclear translocation. The N-terminal (EGFP-STAT6) fusion protein appeared to be more active than the C-terminal fusion. In HEK-293 cells both fusion proteins formed fluorescent nuclear foci following IL-4 stimulation, but in HeLa cells nuclear accumulation was homogeneous. Stimulation of the NF-kappaB pathway through TNFalpha treatment, or expression of p65-EGFP fusion protein, repressed both basal STAT6-dependent transcriptional activity and the extent of activation in response to IL-4. This indicates a novel mechanism of inhibition of STAT6 signalling by NF-kappaB activation. (C) 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
Author(s): Nelson G, Wilde GJC, Spiller DG, Sullivan E, Unitt JF, White MRH
Publication type: Article
Publication status: Published
Journal: FEBS Letters
Print publication date: 04/12/2002
Online publication date: 09/11/2002
ISSN (print): 0014-5793
ISSN (electronic): 1873-3468
Publisher: John Wiley & Sons Ltd
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