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Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems

Lookup NU author(s): Alice Eberhardt, Dr Ling Juan Wu, Professor Jeff ErringtonORCiD, Professor Waldemar Vollmer, Dr Jan-Willem Veening


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The molecular mechanisms underlying cell growth, cell division and pathogenesis in Streptococcus pneumoniae are still not fully understood. Single-cell methodologies are potentially of great value to investigate S. pneumoniae cell biology. Here, we report the construction of novel plasmids for single and double cross-over integration of functional fusions to the gene encoding a fast folding variant of the green fluorescent protein (GFP) into the S. pneumoniae chromosome. We have also established a zinc-inducible system for the fine control of gfp-fusion gene expression and for protein depletion experiments in S. pneumoniae. Using this novel single cell toolkit, we have examined the cellular localization of the proteins involved in the essential process of choline decoration of S. pneumoniae teichoic acid. GFP fusions to LicA and LicC, enzymes involved in the activation of choline, showed a cytoplasmic distribution, as predicted from their primary sequences. A GFP fusion to the choline importer protein LicB showed clear membrane localization. GFP fusions to LicD1 and LicD2, enzymes responsible for loading of teichoic acid subunits with choline, are also membrane-associated, even though both proteins lack any obvious membrane spanning domain. These results indicate that the decoration of teichoic acid by the LicD enzymes is a membrane-associated process presumably occurring at lipid-linked teichoic acid precursors.

Publication metadata

Author(s): Eberhardt A, Wu LJ, Errington J, Vollmer W, Veening J-W

Publication type: Article

Publication status: Published

Journal: Molecular Microbiology

Year: 2009

Volume: 74

Issue: 2

Pages: 395-408

ISSN (print): 0950-382X

ISSN (electronic): 1365-2958

Publisher: Wiley-Blackwell Publishing Ltd.


DOI: 10.1111/j.1365-2958.2009.06872.x


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Funder referenceFunder name
Newcastle University
UK Biotechnology and Biological Sciences Research Council
Intra-European Marie-Curie Fellowship
Royal Netherlands Academy of Arts and Sciences
LSHM-CT-2004-512138EUR-INTAFAR project