Browse by author
Lookup NU author(s): Professor Peter Thelwall
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Non-invasive magnetic resonance imaging and spectroscopy techniques have been used to monitor the growth and distribution of Chinese hamster ovary K1 cells growing in a fixed bed bioreactor composed of macroporous carriers. Diffusion-weighted 1H magnetic resonance spectroscopy was used to monitor the volume fraction of the bioreactor occupied by the cells and diffusion-weighted 1H magnetic resonance imaging was used to map cell distribution. The imaging measurements demonstrated that cell growth in the bioreactor was heterogeneous, with the highest cell densities being found at the surface of the carriers. The increase in the volume fraction occupied by the cells during cell growth showed a close correlation with bioreactor ATP content measured using 31P magnetic resonance spectroscopy. These magnetic resonance measurements, in conjunction with measurements of bioreactor glucose consumption, allowed estimation of the specific glucose consumption rate. This declined during the culture, in parallel with medium glucose concentration.
Author(s): Thelwall PE, Brindle KM
Publication type: Article
Publication status: Published
Print publication date: 01/07/1999
ISSN (print): 0920-9069
ISSN (electronic): 1573-0778
Publisher: Springer Netherlands
Altmetrics provided by Altmetric