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Analysis of CHO-K1 cell growth in a fixed bed bioreactor using magnetic resonance spectroscopy and imaging

Lookup NU author(s): Professor Peter Thelwall

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Abstract

Non-invasive magnetic resonance imaging and spectroscopy techniques have been used to monitor the growth and distribution of Chinese hamster ovary K1 cells growing in a fixed bed bioreactor composed of macroporous carriers. Diffusion-weighted 1H magnetic resonance spectroscopy was used to monitor the volume fraction of the bioreactor occupied by the cells and diffusion-weighted 1H magnetic resonance imaging was used to map cell distribution. The imaging measurements demonstrated that cell growth in the bioreactor was heterogeneous, with the highest cell densities being found at the surface of the carriers. The increase in the volume fraction occupied by the cells during cell growth showed a close correlation with bioreactor ATP content measured using 31P magnetic resonance spectroscopy. These magnetic resonance measurements, in conjunction with measurements of bioreactor glucose consumption, allowed estimation of the specific glucose consumption rate. This declined during the culture, in parallel with medium glucose concentration.


Publication metadata

Author(s): Thelwall PE, Brindle KM

Publication type: Article

Publication status: Published

Journal: Cytotechnology

Year: 1999

Volume: 30

Issue: 1/3

Pages: 121-132

Print publication date: 01/07/1999

ISSN (print): 0920-9069

ISSN (electronic): 1573-0778

Publisher: Springer Netherlands

URL: http://dx.doi.org/10.1023/A:1008039011960

DOI: 10.1023/A:1008039011960


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