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Lookup NU author(s): Dr Richard Daniel,
Dr Yoshikazu Kawai,
Professor Jeff Errington FRS
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Little is known about the organization or proteins involved in membrane-associated replication of prokaryotic genomes. Here we show that the actin-like MreB cytoskeleton of the distantly related bacteria Escherichia coli and Bacillus subtilis is required for efficient viral DNA replication. Detailed analyses of B. subtilis phage phi 29 showed that the MreB cytoskeleton plays a crucial role in organizing phage DNA replication at the membrane. Thus, phage double-stranded DNA and components of the phi 29 replication machinery localize in peripheral helix-like structures in a cytoskeleton-dependent way. Importantly, we show that MreB interacts directly with the phi 29 membrane-protein p16.7, responsible for attaching viral DNA at the cell membrane. Altogether, the results reveal another function for the MreB cytoskeleton and describe a mechanism by which viral DNA replication is organized at the bacterial membrane.
Author(s): Muñoz-Espín D, Daniel R, Kawai Y, Carballido-López R, Castilla-Llorente V, Errington J, Meijer WJJ, Salas M
Publication type: Article
Publication status: Published
Journal: Proceedings of the National Academy of Sciences
ISSN (print): 0027-8424
ISSN (electronic): 1091-6490
Publisher: National Academy of Sciences
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