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Silicon Surface Nanostructuring for Covalent Immobilization of Biomolecules

Lookup NU author(s): Dr Celia Rogero, Dr Benjamin Chaffey, Dr Ben Horrocks, Professor Andrew HoultonORCiD, Professor Jeremy LakeyORCiD


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We present a straightforward strategy to control the average distance of immobilized biomolecules on silicon surfaces. We exploit the reaction taking place between the amino residues within the biomolecules (lysine groups of proteins or the N-terminus of oligomers of peptide nucleic acid, PNA) and the aldehyde-terminated groups presented in a mixed aldehyde/alkyl organic monolayer on a silicon surface. The mixed monolayers were prepared by a thermal reaction of hydrogen-terminated Si(111) with a mixture of undecene and undecenyl-aldehyde. We quantitatively evaluate the surface concentration of aldehyde in the monolayer by atomic force microscopy and an intensity analysis of core level X-ray photoemission spectroscopy peaks. These complementary techniques show that the surface density of the reactive terminal groups reflects the mole fraction of aldehyde in the reactive solution used to modify the silicon surface. The further immobilization of proteins or peptide nucleic acids on the monolayer shows that the density of biomolecules reproduces the aldehyde surface density, which indicates a specific covalent attachment and a negligible nonspecific adsorption. The proposed procedure makes possible to control the average distance of the immobilized active biomolecules on the silicon surface, which could be of great relevance for applications in the interdisciplinary field of biosensors.

Publication metadata

Author(s): Rogero C, Chaffey BT, Mateo-Martí E, Sobrado JM, Horrocks BR, Houlton A, Lakey JH, Briones C, Martín-Gago JA

Publication type: Article

Publication status: Published

Journal: Journal of Physical Chemistry C

Year: 2008

Volume: 112

Issue: 25

Pages: 9308-9314

ISSN (print): 1932-7447

ISSN (electronic): 1932-7455

Publisher: American Chemical Society


DOI: 10.1021/jp801543p


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