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Development of Specific Oligonucleotide Probes to Detect Vibrio Species A Preliminary Study

Lookup NU author(s): Umberto Molini, Narut Thanantong, Dr Olivier Sparagano


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Many species of Vibrio are responsible for diseases in marine organisms and for economic losses to the aquaculture industry. The aim of this preliminary study was to obtain species-specific DNA zones to be used as potential probes from a phylogenetic analysis of the 23S ribosomal RNA (rRNA) gene of different Vibrio species from marine and human organisms. Species-specific probes were identified for V parahaemolyticus, V. fortis, V. splendidus, and for two clusters of taxonomically related species, namely V. harveyi/campbelli and V. lentus/aestuarianus. A reverse line blot assay showed that the designed probes can specifically detect the different Vibrio species, thereby proving that these probes can be used to evaluate the presence of pathogenic and nonpathogenic Vibrio species in the sea and in marine organisms to assist in the investigation of environmental risks.

Publication metadata

Author(s): Molini U, Thanantong N, Giangaspero A, Sparagano OAE

Editor(s): Sparagano, O; Maillard, JC; Figueroa , JV

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: Animal Biodiversity and Emerging Diseases: Prediction and Prevention

Year of Conference: 2008

Pages: 312-314

ISSN: 0077-8923 (print) 1749-6632 (online)

Publisher: Wiley-Blackwell Publishing, Inc.


DOI: 10.1196/annals.1428.049

Library holdings: Search Newcastle University Library for this item

Series Title: Annals of the New York Academy of Sciences

ISBN: 9781573317146