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The Effect of Cryopreservation on Umbilical Cord Blood Endothelial Progenitor Cell Differentiation

Lookup NU author(s): Professor Stephen Proctor, Professor Anne Dickinson

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Abstract

Endothelial progenitor cells (EPCs) has been shown to be present in Umbilical cord blood (UCB) in addition to hematopoietic stein cells. Cryopreservation is the accepted method for long-term storage of UCB. However, whether EPCs can be derived front cryopreserved UCB samples is unclear. The aim of this study was to investigate the differentiation potential of EPCs from cryopreserved CB samples. CD34(+) cells were isolated from fresh or frozen and thawed UCB using magnetic beads. Cells were then cultured on fibronectin-coated plates containing endothelial differentiation medium. After 4-5 weeks in culture, endothelial-like cells were generated from fresh UCB samples, but not cryopreserved UCB samples. Examining this further, both fresh and frozen/thawed UCB MNCs were stained with Annexin V-PE and 7-actinomycin D (7-AAD) using flow cytometry. We found that there were a significant number of apoptotic cells in cryopreserved UCB samples compared to fresh UCB samples. In conclusion, cryopreservation induced UCB cell apoptosis and impaired EPC differentiation.

Publication metadata

Author(s): Lu XM, Proctor SJ, Dickinson AM

Publication type: Article

Publication status: Published

Journal: Cell Transplantation

Year: 2008

Volume: 17

Issue: 12

Pages: 1423-1428

ISSN (print): 0963-6897

ISSN (electronic): 1555-3892

Publisher: Cognizant Communication Corp.

URL: http://dx.doi.org/10.3727/096368908787648155

DOI: 10.3727/096368908787648155

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