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Measurement of affective state during chronic nicotine treatment and withdrawal by affective taste reactivity in mice: The role of endocannabinoids

Lookup NU author(s): Victoria Wing, Dr Mohammed Shoaib


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Despite tobacco being highly addictive, it is unclear if nicotine has significant affective properties. To address this, we studied taste reactions to gustatory stimuli, palatable sucrose and unpalatable quinine, which are believed to reflect ongoing affective state. Taste reactivity was assessed during chronic nicotine administration and spontaneous withdrawal and the role of the endogenous cannabinoids was also investigated. C57BL6J mice were implanted with intraoral fistula to allow passive administration of solutions. In the first study, taste reactivity was tracked throughout chronic vehicle or nicotine (12 mg/kg/day) infusion via osmotic minipumps and spontaneous withdrawal following removal of minipumps. In the second study, the endocannabinoid CBI-receptor antagonist AM251 (1, 3 and 10mg/kg, intraperitoneal) or vehicle was acutely administered before taste reactivity measurement during chronic nicotine administration. Chronic nicotine treatment and spontaneous withdrawal did not influence taste reactions to sucrose or quinine. AM251 decreased positive reactions to sucrose and increased negative reactions to quinine. The effects of AM251 were respectively attenuated and enhanced in nicotine infused mice. These results suggest chronic nicotine exposure and withdrawal has no apparent affective sequelae, as probed by taste reactivity, and thus may not explain the difficulty tobacco-users have in achieving abstinence. In contrast, endocannabinoids elevate affective state in drug-naive animals and changes in endogenous endocannabinoid tone may underlie compensations in affective state during chronic nicotine exposure. (C) 2009 Elsevier Inc. All rights reserved.

Publication metadata

Author(s): Wing VC, Cagniard B, Murphy NP, Shoaib M

Publication type: Conference Proceedings (inc. Abstract)

Publication status: Published

Conference Name: Biochemical Pharmacology: Symposium on Nicotinic Acetylcholine Receptors as Therapeutic Targets

Year of Conference: 2009

Pages: 825-835

ISSN: 0006-2952

Publisher: Elsevier Inc.


DOI: 10.1016/j.bcp.2009.06.017

Library holdings: Search Newcastle University Library for this item

ISBN: 18732968