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Potential use of DNA adducts to detect mutagenic compounds in soil

Lookup NU author(s): Dr Ian Singleton


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In this study, three different soils with contrasting features, spiked with 300 mg benzo[a]pyrene (BaP)/kg dry soil, were incubated at 20 degrees C and 60% water holding capacity for 540 days. At different time points, BaP and DNA were extracted and quantified, and DNA adducts were quantified by P-32-postlabelling. After 540 days incubation, 69.3, 81.6 and 83.2% of initial BaP added remained in Cruden Bay, Boyndie and Insch soils, respectively. Meanwhile, a significantly different amount of DNA-BaP adducts were found in the three soils exposed to BaP over time. The work demonstrates the concept that DNA adducts can be detected on DNA extracted from soil. Results suggest the technique is not able to directly reflect bioavailability of BaP transformation products. However, this new method provides a potential way to detect mutagenic compounds in contaminated soil and to assess the outcomes of soil remediation. Crown Copyright (C) 2008 Published by Elsevier Ltd. All rights reserved.

Publication metadata

Author(s): Hua GX, Lyons B, Killham K, Singleton I

Publication type: Article

Publication status: Published

Journal: Environmental Pollution

Year: 2009

Volume: 157

Issue: 3

Pages: 916-921

ISSN (print): 0269-7491

ISSN (electronic): 1873-6424

Publisher: Pergamon


DOI: 10.1016/j.envpol.2008.10.026


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