ePrints

Synthesis and characterization of size-selective nanoporous polymeric adsorbents for blood purification

Lookup NU author(s): Dr Nicholas Hoenich

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

Crosslinked polystyrene co-divinylbenzene adsorbent microspheres with median diameters between 40 and 300 mu m, with an enhanced proportion of mesopores in the range 4-10 nm, have been synthesized using a membrane emulsification technique. The aim was to develop an adsorbent for the selective removal of middle molecular weight uraemic toxins (size range 0.5-20 kDa), e.g. beta(2)-microglobulin (11.8 kDa), whilst size-excluding larger blood proteins, e.g. serum albumin (MW 69 kDa). Using inverse size exclusion chromatography, the role of size exclusion has been demonstrated using hen egg lysozyme (a surrogate for beta(2)-microglobulin) and human serum albumin as model proteins for batch adsorption studies. The adsorbent sample possessing a nanoporous structure with pores predominantly smaller than 10 nm successfully size-excludes serum albumin whilst displaying significant adsorption capacity for the middle molecular weight lysozyme (14.3 kDa). (C) 2009 Elsevier B.V. All rights reserved.

Publication metadata

Author(s): Malik DJ, Webb C, Holdich RG, Ramsden JJ, Warwick GL, Roche I, Williams DJ, Trochimczuk AW, Dale JA, Hoenich NA

Publication type: Article

Publication status: Published

Journal: Separation and Purification Technology

Year: 2009

Volume: 66

Issue: 3

Pages: 578-585

ISSN (print): 1383-5866

ISSN (electronic): 1873-3794

Publisher: Pergamon

URL: http://dx.doi.org/10.1016/j.seppur.2009.01.016

DOI: 10.1016/j.seppur.2009.01.016

Altmetrics

Funding

Share