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Lookup NU author(s): Professor Janet Quinn
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Stress responses are important for the virulence of the major fungal pathogen of humans, Candida albicans. In this study we employed a 2-DE approach to examine the impact of exposure to peroxide (5 mM H2O2), salt (300 mM NaCl) or cadmium stress (0.5 mM Cd2+) upon the C. albicans proteome. Highly reproducible changes in the C. albicans proteome were observed in response to each stress condition. Significantly more proteins were up-regulated in response to cadmium (77) than to the salt (35) or peroxide stresses (35). These proteomic changes displayed minimal overlap with those observed in the transcriptome under equivalent conditions and, importantly, revealed functional categories that respond to stress at the protein level but not the transcript level. Six proteins were up-regulated by all three conditions: Adh1, Atp2, Cip1, Eft2, Ssa1 and Ssb1, which is consistent with the concept that a core stress response exists in C. albicans. This is the first time that a fungal core stress response has been defined at the proteomic level. We have also shown that the Hog1 stress-activated mitogen-activated protein kinase, which is activated in response to the stresses examined in this study, makes a major contribution to the C. albicans stress proteome.
Author(s): Yin ZK, Stead D, Walker J, Selway L, Smith DA, Brown AJP, Quinn J
Publication type: Article
Publication status: Published
Journal: Proteomics
Year: 2009
Volume: 9
Issue: 20
Pages: 4686-4703
ISSN (print): 1615-9853
ISSN (electronic): 1615-9861
Publisher: Wiley - V C H Verlag GmbH & Co. KGaA
URL: http://dx.doi.org/10.1002/pmic.200800958
DOI: 10.1002/pmic.200800958
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