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First structural characterization of Burkholderia vietnamiensis lipooligosaccharide from cystic fibrosis-associated lung transplantation strains

Lookup NU author(s): Christine Aldridge, Professor Kate Gould, Emeritus Professor Paul Corris, Dr Anjam Khan, Professor Anthony De Soyza, Professor Antonio Molinaro

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Abstract

This is the first structural elucidation of the lipooligosaccharide (LOS) endotoxin isolated from Burkholderia vietnamiensis, a clinically important member of Burkholderia cepacia complex, a group of over 10 opportunistic species that are highly problematic in cystic fibrosis. We have characterized a novel LOS structure extracted from two clonal strains of B. vietnamiensis isolated from a cystic fibrosis patient who underwent lung transplantation. Strains were selected from the pretransplantation and post-transplantation periods and endotoxin was extracted. Subsequent analysis interestingly revealed identical oligosaccharidic sequences, but variation in lipid A moieties. Further, both LOS fractions were tested for their immunostimulatory activity on human myelomonocytic U937 cells and for signaling on an HEK293 cell line stably expressing both TLR 4 and MD-2. We observed an increase in lipid A acylation and a resultant increase in biological activity in bio-reporter assays of TNF-alpha secretion in the post-transplantation strain.


Publication metadata

Author(s): Ierano T, Silipo A, Sturiale L, Garozzo D, Bryant C, Lanzetta R, Parrilli M, Aldridge C, Gould FK, Corris PA, Khan CMA, De Soyza A, Molinaro A

Publication type: Article

Publication status: Published

Journal: Glycobiology

Year: 2009

Volume: 19

Issue: 11

Pages: 1214-1223

ISSN (print): 0959-6658

ISSN (electronic): 1460-2423

Publisher: Oxford University Press

URL: http://dx.doi.org/10.1093/glycob/cwp112

DOI: 10.1093/glycob/cwp112


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