Browse by author
Lookup NU author(s): Dr Rachel Lakey, Emeritus Professor Tim Cawston
Full text for this publication is not currently held within this repository. Alternative links are provided below where available.
Objective. To investigate the effect of SSZ on the release of GAG and collagen fragments from bovine nasal cartilage and MMP and ADAMTS (a disintegrin and metalloproteinase domain with thrombospondin motifs) proteinases from human articular chondrocytes (HACs) stimulated with IL-1 alpha and oncostatin M (OSM). Methods. SSZ was added to bovine nasal explant cultures stimulated to resorb with IL-1 alpha and OSM, and the release of GAG and collagen has been determined. Collagenolytic activity was measured using the radio-labelled collagen bioassay. HACs were treated with IL-1 alpha and OSM with and without SSZ, and MMP-1 and -13 and ADAMTS-4 and -5 were measured for protein and gene expression by ELISA and RT-PCR, respectively. Results. SSZ blocked GAG and collagen fragment release from bovine cartilage, and reduced active and total collagenase activity in a dose-dependent manner. SSZ transcriptionally blocked MMP-1, -13 and ADAMTS-4, and reduced the protein levels of MMP-1 and -13 in a dose-dependent manner following stimulation of HACs with IL-1 alpha and OSM. Conclusion. This study shows for the first time that SSZ blocks release of proteoglycan and collagen fragments from resorbing cartilage and lowers the levels of proteoglycan and collagen-degrading enzymes. These results indicate that in addition to acting as an anti-inflammatory agent, SSZ may have a therapeutic role in protecting cartilage from damage in OA.
Author(s): Lakey RL, Cawston TE
Publication type: Article
Publication status: Published
Journal: Rheumatology
Year: 2009
Volume: 48
Issue: 10
Pages: 1208-1212
ISSN (print): 1462-0324
ISSN (electronic): 1462-0332
Publisher: Oxford University Press
URL: http://dx.doi.org/10.1093/rheumatology/kep236
DOI: 10.1093/rheumatology/kep236
Altmetrics provided by Altmetric
