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Impact of plasmid size on cellular oxygen demand in Escherichia coli

Lookup NU author(s): Dr Ronan O'Kennedy

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Abstract

Escherichia coli DH5a harbouring either pBGS18, a 4.4 kb pUC-based plasmid, or pQR150, a 20 kb derivative of pBGS18, were grown in glucose-limited chemostat culture to investigate the effects of plasmid size and recombinant protein production on oxygen demand. Under non-induced conditions, where no recombinant protein was expressed, the cellular oxygen demand of cells harbouring pBGS18 and pQR150 was not significantly different, mean specific oxygen uptake rate (OUR) being 0.75±0.24 and 0.78±0.32 mmol/h per mg of plasmid respectively. Under isopropyl b-D-thiogalactoside-induced conditions, where recombinant protein was expressed, cells harbouring pBGS18 demonstrated a statistically insignificant 37% increase in mean specific OUR while those harbouring pQR150 demonstrated a statistically significant 415% increase. It is concluded that plasmid size does not significantly affect oxygen demand and that increasing oxygen demand observed with increasing plasmid size is due to production of recombinant protein.

Publication metadata

Author(s): Kay A, O'Kennedy RD, Keshavarz-Moore E

Publication type: Article

Publication status: Published

Journal: Biotechnology and Applied Biochemistry

Year: 2003

Volume: 38

Issue: 1

Pages: 1-7

ISSN (print): 0885-4513

ISSN (electronic): 1470-8744

Publisher: Portland Press Ltd.

URL: http://dx.doi.org/10.1042/BA20030022

DOI: 10.1042/BA20030022

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