ePrints

Rapid quantitation and monitoring of plasmid DNA using an ultrasensitive DNA binding dye

Lookup NU author(s): Dr Ronan O'Kennedy

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.

Abstract

A sensitive fluorescence-based method for monitoring plasmid DNA during production was investigated. This simple method of assaying for plasmid DNA allows rapid monitoring of plasmid yields from a recombinant Escherichia coli fed-batch fermentation. The assay has several advantages over traditional methods of plasmid DNA measurement. The fluorescent dye is highly specific and can measure total plasmid DNA concentration in about 5 min. The assay is sensitive over a wide range of plasmid concentrations of between 15 and 280 ng/mL, even in the presence of impurities that occur within alkaline lysate preparations. The technique can also be applied to monitoring fermentation and downstream purification steps.

Publication metadata

Author(s): Noites IS, O'Kennedy RD, Levy MS, Abidi N, Keshavarz-Moore E

Publication type: Article

Publication status: Published

Journal: Biotechnology and Bioengineering

Year: 1999

Volume: 66

Issue: 3

Pages: 195-201

ISSN (print): 0006-3592

ISSN (electronic): 1097-0290

Publisher: John Wiley & Sons, Inc

URL: http://dx.doi.org/10.1002/(SICI)1097-0290(1999)66:3<195::AID-BIT8>3.0.CO;2-6

DOI: 10.1002/(SICI)1097-0290(1999)66:3<195::AID-BIT8>3.0.CO;2-6

Altmetrics

Share