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Prostaglandin Receptor EP1 and EP2 Site in Guinea Pig Bladder Urothelium and Lamina Propria

Lookup NU author(s): Professor James Gillespie


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Purpose: Urothelium has 2 main functions. It is a barrier to urine and has a sensory role. In response to stretch urothelium releases various substances that modulate afferent nerve activity. Recent data on the localization of cyclooxygenase type 1, the enzyme responsible for prostaglandin production, suggests that prostaglandin may have complex local action.. Materials and Methods: The bladders of 7 guinea pigs were stained for prostaglandin receptors type 1 and 2, and costained for vimentin and cyclooxygenase I. Results: Prostaglandin receptor type 1 staining was seen in urothelial cells and in the suburothelium. Urothelial staining, which was often punctuate and weak, was detected in all urothelial cell layers, including suburothelial cells. In contrast, strong prostaglandin receptor type 2 staining was seen in the urothelium and in suburothelial cells. Cyclooxygenase I was absent in interstitial cells and umbrella cells with the highest concentration in the basal cell layer. Conclusions: Interstitial cells express prostaglandin receptor types 1 and 2, indicating that they can respond to prostaglandin. Umbrella cells do not express cyclooxygenase I. Cyclooxygenase I was present in basal urothelial cells, making them a possible site of prostaglandin synthesis. Thus, prostaglandin produced by urothelium may target prostaglandin receptor types 1 and 2 in the urothelium and suburothelium. Therefore prostaglandin is hypothesized to have a role in signal regulation in the bladder wall.

Publication metadata

Author(s): Rahnama'i MS, van Koeveringe GA, Essers PB, de Wachter SGG, de Vente J, van Kerrebroeck PE, Gillespie JI

Publication type: Article

Publication status: Published

Journal: Journal of Urology

Year: 2010

Volume: 183

Issue: 3

Pages: 1241-1247

Print publication date: 22/01/2010

ISSN (print): 0022-5347

ISSN (electronic): 1527-3792

Publisher: Elsevier Inc.


DOI: 10.1016/j.juro.2009.11.004


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