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Expression and functional analyses of liver expressed antimicrobial peptide-2 (LEAP-2) variant forms in human tissues

Lookup NU author(s): Dr Alison Howard, Dr Claire Townes, Panagiota Milona, Dr Christopher NileORCiD, Dr Judith Hall

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Abstract

The antimicrobial peptide Liver Expressed Antimicrobial Peptide-2 (LEAP-2) is proposed to function as part of the vertebrate innate immune system. However, the highly conserved nature of the LEAP-2 peptide primary structure among vertebrates Suggests more fundamental physiological roles. RT-PCR analyses confirmed expression of LEAP-2 mRNA variants in human gastro-intestinal (GI) epithelial tissues and THP-1 monocytes. Three cDNA products indicative of at least three different spliced transcripts were observed. Translation of the cDNA sequences supported synthesis of transcripts encoding the secreted LEAP-2 peptide and two variants lacking signal sequences Suggesting intracellular localisation. The synthesis and cytoplasmic localisation of LEAP-2 peptides in epithelia Was Supported by immunohistochemical analyses. Functional data Suggested that LEAP-2 is not involved in the physiological response of GI epithelia to iron, nor is it mitogenic for epithelial cells or chemotactic for THP-1 monocytes. However, changes in the LEAP-2 transcript patterns associated with the challenge of THP-1 monocytes with lipopolysaccharide (100 ng/ml) were supportive of the peptides having multiple roles in the innate immune response. (C) 2009 Elsevier Inc. All Fights reserved.


Publication metadata

Author(s): Howard A, Townes C, Milona P, Nile CJ, Michailidis G, Hall J

Publication type: Article

Publication status: Published

Journal: Cellular Immunology

Year: 2010

Volume: 261

Issue: 2

Pages: 128-133

ISSN (print): 0008-8749

ISSN (electronic): 1090-2163

Publisher: Academic Press

URL: http://dx.doi.org/10.1016/j.cellimm.2009.11.010

DOI: 10.1016/j.cellimm.2009.11.010


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