Toggle Main Menu Toggle Search

Open Access padlockePrints

Comparative Effects of Cyfluthrin on Intracellular Protease Activities in Insecticide-Resistant and Susceptible Strains of Musca domestics L.

Lookup NU author(s): Professor Mushtaq Saleem, Dr Richard WilkinsORCiD


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


To elucidate whether insecticide toxicity in insects involves insecticide-induced abnormalities of the intracellular protein catabolic process, we have determined in vivo effect of one of the commonly used synthetic pyrethroid, cyfluthrin on the activities of representative protein catabolising cytoplasmic and lysosomal proteases (responsible for the various stages of the protein degradation cascade and essential for normal cell functioning) in resistant and susceptible strains of Musca domestica. Effect of cyfluthrin was determined at LC50 dose level after 48 hour of treatment in both live and dead adult flies, compared with controls. In susceptible strain, cyfluthrin increased all cytoplasmic proteases in surviving flies (ranging from 16 to 148%) and their elevation was further intensified in dead flies (ranging from 24 to 177%). Of lysosomal proteases only cathepsin H (+18%) and D (-26%) were affected in surviving flies while in dead flies cathepsin B and L were additionally affected. Conversally in resistant flies, cyfluthrin produced mixed responses of increase or decrease in cytoplasmic and lysosomal proteases of surviving flies and this affect was further increased or it adopted the route of readjustment in dead flies. We conclude that cyfluthrin affected almost all proteases and such changes in proteases could play an important role in the development of resistance in M. domestica to insecticides.

Publication metadata

Author(s): Wilkins RM; Saleem MA; Shakoori AR

Publication type: Article

Publication status: Published

Journal: Pakistan Journal of Zoology

Year: 2010

Volume: 42

Issue: 5

Pages: 623-630

Print publication date: 01/10/2010

ISSN (print): 0030-9923

ISSN (electronic):

Publisher: Zoological Society of Pakistan