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A cost-effective, simple and high-throughput method for DNA extraction from insects

Lookup NU author(s): Dr Malgorzata Lagisz, Dr Gordon Port, Dr Kirsten Wolff

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Abstract

We present a high-throughput cost-effective method to extract DNA suitable for polymerase chain reaction (PCR) from insect tissue. The method uses standard 200 μL-deep 96-well plates in which samples are ground, digested and subsequently purified. The test extraction using four different insect species and controlling for potential contamination showed that the method yields good-quantity and quality DNA. PCR with mitochondrial and nuclear primers was reliable. The proposed extraction protocol combines the speed of commercial 96-well plate methods with the economies associated with readily available and cheap laboratory chemicals, consumables and equipment. Therefore, this method is particularly suitable for low-budget research projects and for laboratories with only basic equipment present.

Publication metadata

Author(s): Lagisz M, Port G, Wolff K

Publication type: Article

Publication status: Published

Journal: Insect Science

Year: 2010

Volume: 17

Issue: 5

Pages: 465-470

Print publication date: 05/04/2010

ISSN (print): 1672-9609

ISSN (electronic): 1744-7917

Publisher: Wiley-Blackwell

URL: http://dx.doi.org/10.1111/j.1744-7917.2010.01318.x

DOI: 10.1111/j.1744-7917.2010.01318.x

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