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Sialomucin complex (rat Muc4) transmembrane subunit binds the differentiation marker peanut lectin in the normal rat mammary gland

Lookup NU author(s): Dr Peter Li

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Abstract

Sialomucin complex (SMC, rat Muc4) is a heterodimeric glycoprotein composed of two subunits, the mucin component ASGP-1 and the transmembrane subunit ASGP-2. SMC/Muc4 is highly expressed on the surface of 13762 rat mammary adenocarcinoma cells at approximately 100 times the level found in the lactating mammary gland. Immunocytochemical staining of SMC/Muc4 in the developing rat mammary gland is localized to the apical membrane of the ductal epithelium. This staining pattern is similar to that for peanut lectin, a differentiation marker, which binds to cells expressing the disaccharide Thomsen-Friedenreich or TF antigen. Blotting of glycoproteins expressing the TF antigen from mammary tissues with peanut lectin detects a protein matching the migration of ASGP-2. Analysis of immunoprecipitated SMC/Muc4 by peanut lectin blotting shows that the TF antigen is abundantly present on the ASGP-2 subunit, hence the similarity of staining pattern with SMC/Muc4 antisera and peroxidase-conjugated lectin in mammary tissues. The TF antigen is also present on ASGP-2 of SMC/Muc4 produced by confluent cultures of Rama 37 rat mammary epithelial stem cells after their induction to an alveolar-like phenotype with prolactin. These results indicate that the TF antigen is present on the ASGP-2 transmembrane subunit of SMC/Muc4 from phenotypically normal tissues and cells, in contrast to malignant cells whose peanut lectin-binding disaccharide is located on ASGP-1.

Publication metadata

Author(s): Li P, Price-Schiavi SA, Rudland PS, Carraway KL

Publication type: Article

Publication status: Published

Journal: Journal of Cellular Physiology

Year: 2001

Volume: 186

Issue: 3

Pages: 397-405

ISSN (print): 0021-9541

ISSN (electronic): 1097-4652

Publisher: John Wiley & Sons Inc.

URL: http://dx.doi.org/10.1002/1097-4652(2000)9999:999<000::AID-JCP1037>3.0.CO;2-V

DOI: 10.1002/1097-4652(2000)9999:999<000::AID-JCP1037>3.0.CO;2-V

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