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Lookup NU author(s): Dr Kevin WaldronORCiD, Harriet Denton, Professor Nigel Robinson, Dr Jennifer Cavet
Salmonella enterica sv. typhimurium (S. enterica sv. Typhimurium) has two metal-transporting P-1-type ATPases whose actions largely overlap with respect to growth in elevated copper. Mutants lacking both ATPases over-accumulate copper relative to wild-type or either single mutant. Such duplication of ATPases is unusual in bacterial copper tolerance. Both ATPases are under the control of MerR family metal-responsive transcriptional activators. Analyses of periplasmic copper complexes identified copper-CueP as one of the predominant metal pools. Expression of cueP was recently shown to be controlled by the same metal-responsive activator as one of the P1-type ATPase genes (copA), and copper-CueP is a further atypical feature of copper homeostasis in S. enterica sv. Typhimurium. Elevated copper is detected by a reporter construct driven by the promoter of copA in wild-type S. enterica sv. Typhimurium during infection of macrophages. Double mutants missing both ATPases also show reduced survival inside cultured macrophages. It is hypothesized that elevated copper within macrophages may have selected for specialized copper-resistance systems in pathogenic microorganism such as S. enterica sv. Typhimurium.
Author(s): Osman D, Waldron KJ, Denton H, Taylor CM, Grant AJ, Mastroeni P, Robinson NJ, Cavet JS
Publication type: Article
Publication status: Published
Journal: Journal of Biological Chemistry
Year: 2010
Volume: 285
Issue: 33
Pages: 25259-25268
Print publication date: 01/08/2010
Date deposited: 10/06/2013
ISSN (print): 0021-9258
ISSN (electronic): 1083-351X
Publisher: American Society for Biochemistry and Molecular Biology
URL: http://dx.doi.org/10.1074/jbc.M110.145953
DOI: 10.1074/jbc.M110.145953
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