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Effects of experimental challenge of ewes with Mannheimia haemolytica on subsequent milk composition

Lookup NU author(s): Professor Ilias Kyriazakis

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Abstract

The objective was to describe the physicochemical changes during the early phase of subclinical mastitis and to associate them with pathological findings. A Mannheimia haemolytica strain was deposited into one teat duct of 25 ewes and the clinical, bacteriological, cytological, physicochemical (pH, milk composition), gross-pathological and histological findings were subsequently recorded. The organism was consistently isolated from samples of teat duct material (140/150) but not from mammary secretion (50/150). California Mastitis Test (CMT) scores increased (> 1) and remained high (143/150 samples) after challenge; polymorphonuclear neutrophils (PMN) predominated in milk films, but the proportion of lymphocytes and macrophages progressively increased. Increased pH values (> 7.0) were recorded in the mammary secretion from the challenged side. Furthermore, content of fat, total proteins and lactose therein decreased markedly. Histological changes (leucocytic infiltration, destruction of epithelial cells) were observed in the mammary parenchyma of the ewes. The present results confirm that the reduction of milk constituents is the effect of cellular damage and can occur soon after infection.

Publication metadata

Author(s): Fragkou IA, Solomakos N, Dagleish MP, Cripps PJ, Papaioannou N, Boscos CM, Ververidis HN, Billinis C, Orfanou DC, Govaris A, Kyriazakis I, Fthenakis GC

Publication type: Article

Publication status: Published

Journal: Journal of Dairy Research

Year: 2008

Volume: 75

Issue: 3

Pages: 340-346

ISSN (print): 0022-0299

ISSN (electronic): 1469-7629

Publisher: Cambridge University Press

URL: http://dx.doi.org/10.1017/S0022029908003440

DOI: 10.1017/S0022029908003440

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