Toggle Main Menu Toggle Search

Open Access padlockePrints

A temperature-sensitive mutation in the Arabidopsis thaliana phosphomannomutase gene disrupts protein glycosylation and triggers cell death

Lookup NU author(s): Professor Christine Foyer

Downloads

Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


Abstract

Eukaryotic phosphomannomutases (PMMs) catalyze the interconversion of mannose 6-phosphate to mannose 1-phosphate and are essential to the biosynthesis of GDP-mannose. As such, plant PMMs are involved in ascorbic acid (AsA) biosynthesis and N-glycosylation. We report on the conditional phenotype of the temperature-sensitive Arabidopsis thaliana pmm-12 mutant. Mutant seedlings were phenotypically similar to wild type seedlings when grown at 16-18 °C but died within several days after transfer to 28 °C. This phenotype was observed throughout both vegetative and reproductive development. Protein extracts derived from pmm-12 plants had lower PMM protein and enzyme activity levels. In vitro biochemical analysis of recombinant proteins showed that the mutant PMM protein was compromised in its catalytic efficiency (K cat/Km). Despite significantly decreased AsA levels in pmm-12 plants, AsA deficiency could not account for the observed phenotype. Since, at restrictive temperature, total glycoprotein patterns were altered and glycosylation of protein-disulfide isomerase was perturbed, we propose that a deficiency in protein glycosylation is responsible for the observed cell death phenotype. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.


Publication metadata

Author(s): Hoeberichts FA, Vaeck E, Kiddle G, Coppens E, Van De Cotte B, Adamantidis A, Ormenese S, Foyer CH, Zabeau M, Inze D, Perilleux C, Van Breusegem F, Vuylsteke M

Publication type: Article

Publication status: Published

Journal: Journal of Biological Chemistry

Year: 2008

Volume: 283

Issue: 9

Pages: 5708-5718

ISSN (print): 0021-9258

ISSN (electronic): 1083-351X

Publisher: American Society for Biochemistry and Molecular Biology, Inc.

URL: http://dx.doi.org/10.1074/jbc.M704991200

DOI: 10.1074/jbc.M704991200

PubMed id: 18086684


Altmetrics

Altmetrics provided by Altmetric


Actions

Find at Newcastle University icon    Link to this publication


Share