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Lookup NU author(s): Dr Nikolaos Papageorgiou
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Neuronal models for Alzheimer's disease research frequently have limitations as a result of their nonhuman origin and/or transformed state. Here we examined the potential of readily accessible neural crest-derived human epidermal melanocytes isolated from elderly individuals as a model system for Alzheimer's disease research. The amyloidogenic isoforms of amyloid precursor protein (APP; isoforms APP751/770) and amyloid beta (Aβ) 1-40 were detected in epidermal melanocytes using immunocytochemistry and western blotting. Incubation of epidermal melanocytes with aggregated Aβ1-40 peptide caused a concentration-dependent reduction in cell viability, whereas age-matched dermal fibroblasts remained unaffected. These findings suggest that epidermal melanocytes from elderly donors are capable of amyloidogenesis and are sensitive to Aβ1-40 cytotoxicity. Thus, these cells may provide a readily accessible human cell model for Alzheimer's disease research. © 2008 Wolters Kluwer Health|Lippincott Williams & Wilkins.
Author(s): Papageorgiou N, Carpenter E, Scally AJ, Tobin DJ
Publication type: Article
Publication status: Published
ISSN (print): 0959-4965
ISSN (electronic): 1473-558X
Publisher: Lippincott Williams & Wilkins
PubMed id: 18978644
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