Toggle Main Menu Toggle Search

Open Access padlockePrints

Second-order schedules of nicotine reinforcement in rats: effect of AM251

Lookup NU author(s): Dr Victoria Wing, Dr Mohammed Shoaib


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


The endocannabinoid system has been implicated in the motivational effects of nicotine and nicotine-associated stimuli but the neural circuitry underlying tobacco addiction is not fully characterised. The present study aimed to establish a second-order schedule of nicotine reinforcement to compare the role of the endocannabinoid system in nicotine- and cue-maintained responding. The male rats were successfully trained to respond on a second-order schedule [FR5 (FR5: S) or FI 10' (FR3: S)] under which presentation of the CS (brief light oscillation) was intermittently reinforced by nicotine (0.03 mg/kg/infusion). The relative contribution of nicotine and the CS towards responding was then compared. Nicotine and the CS were only able to independently maintain responding to similar level under the [FI 10' (FR3: S)] schedule, which was subsequently employed to examine the effects of the selective CB1 receptor antagonist AM251. AM251 (0.1, 0.3 and 1 mg/kg, intraperitoneal [i.p.]) was used to examine the role of endocannabinoids in responding under the second-order schedule and responding maintained by independent presentation of nicotine and the CS. Responding under the second-order schedule was dose-dependently attenuated by AM251, whereas responding for independent presentation of nicotine and the CS was not affected. The establishment of second-order schedules of nicotine reinforcement in rodents highlighted the utility of such schedules for investigation of the neurobiology that underlies nicotine- and cue-maintained behaviour. Additionally, the role of CB1 receptors in nicotine-motivated behaviours was extended to those controlled under a second-order schedule.

Publication metadata

Author(s): Wing VC, Shoaib M

Publication type: Article

Publication status: Published

Journal: Addiction Biology

Year: 2010

Volume: 15

Issue: 4

Pages: 393-402

Print publication date: 10/03/2010

ISSN (print): 1355-6215

ISSN (electronic): 1369-1600

Publisher: Wiley-Blackwell Publishing Ltd.


DOI: 10.1111/j.1369-1600.2009.00204.x


Altmetrics provided by Altmetric