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Lookup NU author(s): Emeritus Professor John Harris
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A mutant form of ammodytoxin A, a neurotoxic phospholipase A2 from the venom of the long nosed viper Vipera ammodytes ammodytes, was prepared by site-directed mutagenesis, conjugated to a nanogold particle and inoculated into the antero-lateral aspect of one hind limb of female mice. Eight hours later the mice were killed, the soleus muscles of both ipsi- and contra-lateral hind limbs were removed, exposed to a silver enhancing medium and then prepared for transmission electron microscopy. Silver-enhanced particles were subsequently found concentrated in the peri-synaptic area, particularly within the synaptic gutter and the deep synaptic folds, and in many cases had been taken up into the cytoplasm of the terminal boutons of the motor axon. The results suggest that the presynaptic neurotoxicity of snake venom phospholipases A2 involves several components of the neuromuscular apparatus, including intracellular organelles of the motor nerve terminal. © 2009 Elsevier Inc. All rights reserved.
Author(s): Logonder U, Jenko-Pražnikar Z, Scott-Davey T, Pungerčar J, Križaj I, Harris J
Publication type: Article
Publication status: Published
Journal: Experimental Neurology
ISSN (print): 0014-4886
ISSN (electronic): 1090-2430
Publisher: Academic Press
PubMed id: 19631643
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