Toggle Main Menu Toggle Search

Open Access padlockePrints

Ultrastructural evidence for the uptake of a neurotoxic snake venom phospholipase A2 into mammalian motor nerve terminals

Lookup NU author(s): Emeritus Professor John Harris


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


A mutant form of ammodytoxin A, a neurotoxic phospholipase A2 from the venom of the long nosed viper Vipera ammodytes ammodytes, was prepared by site-directed mutagenesis, conjugated to a nanogold particle and inoculated into the antero-lateral aspect of one hind limb of female mice. Eight hours later the mice were killed, the soleus muscles of both ipsi- and contra-lateral hind limbs were removed, exposed to a silver enhancing medium and then prepared for transmission electron microscopy. Silver-enhanced particles were subsequently found concentrated in the peri-synaptic area, particularly within the synaptic gutter and the deep synaptic folds, and in many cases had been taken up into the cytoplasm of the terminal boutons of the motor axon. The results suggest that the presynaptic neurotoxicity of snake venom phospholipases A2 involves several components of the neuromuscular apparatus, including intracellular organelles of the motor nerve terminal. © 2009 Elsevier Inc. All rights reserved.

Publication metadata

Author(s): Logonder U, Jenko-Pražnikar Z, Scott-Davey T, Pungerčar J, Križaj I, Harris J

Publication type: Article

Publication status: Published

Journal: Experimental Neurology

Year: 2009

Volume: 219

Issue: 2

Pages: 591-594

ISSN (print): 0014-4886

ISSN (electronic): 1090-2430

Publisher: Academic Press


DOI: 10.1016/j.expneurol.2009.07.017

PubMed id: 19631643


Altmetrics provided by Altmetric