Toggle Main Menu Toggle Search

Open Access padlockePrints

Molecular and biochemical characterisation of a dual proteolytic system in vine weevil larvae (Otiorhynchus sulcatus Coleoptera: Curculionidae)

Lookup NU author(s): Dr Martin EdwardsORCiD, Professor Angharad MR GatehouseORCiD


Full text for this publication is not currently held within this repository. Alternative links are provided below where available.


The ability of phytophagous insects to utilise the relatively low nitrogen content of plant tissues is typically the limiting factor in their nutritional uptake. In the larval stage, the vine weevil feeds predominantly on root tissues of plants. The root tissue as a whole has low levels of free amino acids, and thus effective hydrolysis of dietary proteins is essential for survival. In contrast to previous reports the present study demonstrates through both molecular and biochemical studies the presence of proteolytic enzymes from two mechanistic classes, cysteine and serine proteases, in the gut of larval vine weevil; with the latter being the predominant form. cDNA clones encoding cathepsin B-like and serine-like sequences were isolated from a gut specific cDNA library; the cathepsin B-like clone has the Cys-His-Asn catalytic triad. However, the sequence showed the replacement of the conserved His His sequence in the "occluding loop" region of the enzyme with Asp His. This may result in a change to the substrate specificity. Two trypsin precursors contained evidence of a signal peptide, activation peptide, and conserved N-termini (IVGG). Other structural features included typical His, Asp. and Set residues of the catalytic amino acid triad indicative of serine proteases, characteristic residues in the substrate-binding pocket, and four pairs of cysteine residues for disulfide bridges. The apparent abundance of the trypsin-like cDNA clones compared to the cathepsin B clones suggests that serine proteases are the predominant form, thus supporting data from the biochemical studies. (C) 2010 Elsevier Ltd. All rights reserved.

Publication metadata

Author(s): Edwards MG, Gatehouse JA, Gatehouse AMR

Publication type: Article

Publication status: Published

Journal: Insect Biochemistry and Molecular Biology

Year: 2010

Volume: 40

Issue: 11

Pages: 785-791

Print publication date: 13/08/2010

ISSN (print): 0965-1748

ISSN (electronic): 1879-0240

Publisher: Pergamon


DOI: 10.1016/j.ibmb.2010.07.005


Altmetrics provided by Altmetric