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The Relationship Between Prostaglandin E Receptor 1 and Cyclooxygenase I Expression in Guinea Pig Bladder Interstitial Cells: Proposition of a Signal Propagation System

Lookup NU author(s): Professor James Gillespie

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Abstract

Purpose: We explored the structural relationship between enzymes producing prostaglandin (cyclooxygenase I) and 1 of the receptor families that respond to prostaglandin (prostaglandin E receptor 1) in the bladder muscle. Materials and Methods: Nine male guinea pigs were sacrificed by cervical dislocation. Bladders were removed and fixed in 4% paraformaldehyde in phosphate buffered saline. Frozen sections (10 mu m) were cut and stained with antibodies to prostaglandin E receptor type 1, cyclooxygenase I and vimentin. Results: Prostaglandin E receptors 1 was identified on smooth muscle cells, and vimentin positive surface muscle and intramuscular interstitial cells. Muscle staining was less intense than on interstitial cells and had a punctuate appearance. Prostaglandin E receptor 1 expression on interstitial cells was highly localized. Discrete regions of intense staining were noted on interstitial cell processes. Cyclooxygenase I was also expressed in muscle interstitial cells. Cyclooxygenase I positive interstitial cells were more prevalent in the muscle bundles of the inner muscle than in the outer muscle layers. Cyclooxygenase I staining was noted on discrete regions of the cell or cell processes. Double staining with prostaglandin E receptor 1 and cyclooxygenase I suggested that cell regions expressing the former are different from those expressing the latter. Conclusions: The discovered arrangement of prostaglandin E receptor 1 and cyclooxygenase I may have the potential to facilitate the propagation of signals in the interstitial cell network. Such a signaling system may have a role in coordinating events, as in bladder pathology, facilitating the global coordinated changes associated with bladder wall remodeling.

Publication metadata

Author(s): Rahnama'i MS, de Wachter SG, van Koeveringe GA, van Kerrebroeck PE, de Vente J, Gillespie JI

Publication type: Article

Publication status: Published

Journal: Journal of Urology

Year: 2011

Volume: 185

Issue: 1

Pages: 315-322

Print publication date: 13/11/2010

ISSN (print): 0022-5347

ISSN (electronic): 1527-3792

Publisher: Elsevier Inc.

URL: http://dx.doi.org/10.1016/j.juro.2010.09.005

DOI: 10.1016/j.juro.2010.09.005

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