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Regulation of peptidoglycan synthesis by outer-membrane proteins

Lookup NU author(s): Manuel Banzhaf, Dr Jacob BiboyORCiD, Katrin Beilharz, Professor Waldemar Vollmer


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Growth of the mesh-like peptidoglycan (PG) sacculus located between the bacterial inner and outer membranes (OM) is tightly regulated to ensure cellular integrity, maintain cell shape, and orchestrate division. Cytoskeletal elements direct placement and activity of PG synthases from inside the cell, but precise spatiotemporal control over this process is poorly understood. We demonstrate that PG synthases are also controlled from outside of the sacculus. Two OM lipoproteins, LpoA and LpoB, are essential for the function, respectively, of PBP1A and PBP1B, the major E. coli bifunctional PG synthases. Each Lpo protein binds specifically to its cognate PBP and stimulates its transpeptidase activity, thereby facilitating attachment of new PG to the sacculus. LpoB shows partial septal localization, and our data suggest that the LpoB-PBP1B complex contributes to OM constriction during cell division. LpoA/ LpoB and their PBP-docking regions are restricted to gamma-proteobacteria, providing models for niche-specific regulation of sacculus growth.

Publication metadata

Author(s): Typas A, Banzhaf M, van den Berg van Saparoea B, Verheul J, Biboy J, Nichols RJ, Zietek M, Beilharz K, Kannenberg K, von Rechenberg M, Breukink E, den Blaauwen T, Gross CA, Vollmer W

Publication type: Article

Publication status: Published

Journal: Cell

Year: 2010

Volume: 143

Issue: 7

Pages: 1097-1109

Print publication date: 23/12/2010

ISSN (print): 0092-8674

ISSN (electronic): 1097-4172

Publisher: Cell Press


DOI: 10.1016/j.cell.2010.11.038


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Funder referenceFunder name
ARRA GM085697-01S1NIH
F31 DE020206-01NIH
HEALTH-F3-2009-223431European Commission
LSHM-CT-2004-512138European Commission
R01 GM036278NIH
R01 GM085697NIH
T32 DE007306NIH