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Lookup NU author(s): Christine Aldridge,
Dr Jonathan Brown,
Dr Phillip AldridgeORCiD
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The flagellar genes in Salmonella enterica are expressed in a temporal hierarchy that mirrors the assembly process itself. The sigma(28)-FlgM regulatory circuit plays a key role in controlling this temporal hierarchy. This circuit ensures that the class 3 genes are expressed only when the hook-basal body (HBB), a key intermediate in flagellar assembly, is complete. In this work, we investigated the role of the sigma(28)-FlgM regulatory circuit in controlling the timing and magnitude of class 3 gene expression using a combination of mathematical modelling and experimental analysis. Analysis of the model predicted that this circuit continuously controls class 3 gene expression in response to HBB abundance. We experimentally validated these predictions by eliminating different components of the sigma(28)-FlgM regulatory system and also by rewiring the transcriptional hierarchy. Based on these results, we conclude that the sigma(28)-FlgM regulatory circuit continuously senses the HBB assembly process and regulates class 3 gene expression and possibly flagellar numbers in response.
Author(s): Saini S, Floess E, Aldridge C, Brown J, Aldridge PD, Rao CV
Publication type: Article
Publication status: Published
Journal: Molecular Microbiology
Print publication date: 17/11/2010
ISSN (print): 0950-382X
ISSN (electronic): 1365-2958
Publisher: Wiley-Blackwell Publishing Ltd.
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