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Lookup NU author(s): Dr Jon Aust, Frances Davison, Farhana Hussain, Dr Ralf KistORCiD, Dr Heiko Peters, Professor Colin Brooks
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Ly49E is an unusual member of the Ly49 family that is expressed on fetal NK cells, epithelial T cells, and NKT cells, but not on resting adult NK cells. Ly49E(bgeo/bgeo) mice in which the Ly49E gene was disrupted by inserting a beta-geo transgene were healthy, fertile, and had normal numbers of NK and T cells in all organs examined. Their NK cells displayed normal expression of Ly49 and other NK cell receptors, killed tumor and MHC class I-deficient cells efficiently, and produced normal levels of IFN-gamma. In heterozygous Ly49E(+/bgeo) mice, the proportion of epidermal T cells, NKT cells, and IL-2-activated NK cells that expressed Ly49E was about half that found in wild-type mice. Surprisingly, although splenic T cells rarely expressed Ly49E, IL-2-activated splenic T cells from Ly49E(bgeo/bgeo) mice were as resistant to growth in G418 as NK cells and expressed similar levels of beta-geo transcripts, suggesting that disruption of the Ly49E locus had increased its expression in these cells to the same level as that in NK cells. Importantly, however, the proportion of G418-resistant heterozygous Ly49E(+/bgeo) cells that expressed Ly49E from the wild-type allele was similar to that observed in control cells. Collectively, these findings demonstrate that Ly49E is not required for the development or homeostasis of NK and T cell populations or for the acquisition of functional competence in NK cells and provide compelling evidence that Ly49E is expressed in a probabilistic manner in adult NK cells and T cells. The Journal of Immunology, 2011, 186: 2013-2023.
Author(s): Aust JG, Gays F, Hussain F, Butcher GW, Kist R, Peters H, Brooks CG
Publication type: Article
Publication status: Published
Journal: Journal of Immunology
Year: 2011
Volume: 186
Issue: 4
Pages: 2013-2023
Print publication date: 15/02/2011
Online publication date: 19/01/2011
Acceptance date: 14/12/2010
ISSN (print): 0022-1767
ISSN (electronic): 1550-6606
Publisher: American Association of Immunologists
URL: http://dx.doi.org/10.4049/jimmunol.1003698
DOI: 10.4049/jimmunol.1003698
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