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Lookup NU author(s): Professor Muzlifah Haniffa, Professor Matthew CollinORCiD
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Blood of both humans and mice contains 2 main monocyte subsets. Here, we investigated the extent of their similarity using a microarray approach. Approximately 270 genes in humans and 550 genes in mice were differentially expressed between subsets by 2-fold or more. More than 130 of these gene expression differences were conserved between mouse and human monocyte subsets. We confirmed numerous of these differences at the cell surface protein level. Despite overall conservation, some molecules were conversely expressed between the 2 species' subsets, including CD36, CD9, and TREM-1. Other differences included a prominent peroxisome proliferatoractivated receptor γ (PPARγ) signature in mouse monocytes, which is absent in humans, and strikingly opposed patterns of receptors involved in uptake of apoptotic cells and other phagocytic cargo between human and mouse monocyte subsets. Thus, whereas human and mouse monocyte subsets are far more broadly conserved than currently recognized, important differences between the species deserve consideration when models of human disease are studied in mice. © 2010 by The American Society of Hematology.
Author(s): Ingersoll M, Spanbroek R, Lottaz C, Gautier E, Frankenberger M, Hoffmann R, Lang R, Haniffa M, Collin M, Tacke F, Habenicht A, Ziegler-Heitbrock L, Randolph G
Publication type: Article
Publication status: Published
Journal: Blood
Year: 2010
Volume: 115
Issue: 3
Pages: e10-e19
Print publication date: 21/01/2010
ISSN (print): 0006-4971
ISSN (electronic): 1528-0020
Publisher: American Society of Hematology
URL: http://dx.doi.org/10.1182/blood-2009-07-235028
DOI: 10.1182/blood-2009-07-235028
PubMed id: 19965649
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